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Test ID: CHIMU Chimerism Transplant No Cell Sort, Varies

Useful For

Determining the relative amounts of donor and recipient cells in a specimen

 

An indicator of bone marrow transplant success

Testing Algorithm

Complete chimerism analysis requires 3 specimens, under 3 separate orders, for the 3 separate tests listed below. These specimens should be submitted when collected. An interpretive report will be provided once all specimens are received.

 

Pre-Transplant:

-CHRGB / Chimerism-Recipient Germline (Pretransplant), Varies

-CHIDB / Chimerism-Donor, Varies

-ADONO / Additional Chimerism Donor (Bill Only), if applicable

 

Post-Transplant:

-CHIMU / Chimerism Transplant No Cell Sort, Varies or CHIMS / Chimerism Transplant Sorted Cells, Varies

 

Billing occurs with the following tests:

Pre-transplant:

-CHRGB / Chimerism-Recipient Germline (Pretransplant), Varies

-ADONO / Additional Chimerism Donor (Bill Only), if applicable

 

Post-Transplant:

-CHIMU / Chimerism Transplant No Cell Sort, Varies

-CHIMS / Chimerism Transplant Sorted Cells, Varies

-SORT1 / Chimerism Cell Sort 1 (Bill Only)

-SORT2 / Chimerism Cell Sort 2 (Bill Only)

 

For more information see Chimerism-Recipient Germline Testing Algorithm

Method Name

Polymerase Chain Reaction (PCR) Amplification/Capillary Electrophoresis

Reporting Name

Chimerism Transplant No Cell Sort

Specimen Type

Varies


Ordering Guidance


This test is for the post-bone marrow transplant evaluation of the presence of donor cells in the post-transplant recipient specimen. For post-bone marrow transplant testing to determine the relative amounts of donor and recipient cells, see CHIMS / Chimerism Transplant Sorted Cells, Varies



Additional Testing Requirements


 



Shipping Instructions


1. Specimen must arrive within 7 days of collection.

2. Collect and package specimen as close to shipping time as possible.



Necessary Information


Specimen Type is required, either as an answer to the Order Questions or on Chimerism Analysis Information (T594) if not ordering electronically. Testing will be delayed if this information is not provided.



Specimen Required


Submit only 1 of the following specimens:

 

Specimen Type: Blood

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 4 mL

Collection Instructions:

1. Only 1 tube is required.

2. Invert several times to mix blood.

3. Label specimen as blood.

4. Send whole blood specimen in original tube. Do not aliquot.

 

Specimen Type: Bone marrow

Container/Tube:

Preferred: Lavender top (EDTA)

Acceptable: Yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Label specimen as bone marrow.

3. Send bone marrow specimen in original tube. Do not aliquot.


Specimen Minimum Volume

Blood: 3 mL
Bone marrow: See Specimen Required
Lesser volumes may be acceptable, depending on white cell count.
Call 800-533-1710 or 507-266-5700 with questions.

Specimen Stability Information

Specimen Type Temperature Time Special Container
Varies Ambient (preferred) 7 days PURPLE OR PINK TOP/EDTA
  Refrigerated  7 days PURPLE OR PINK TOP/EDTA

Clinical Information

Patients who have had donor hematopoietic cells infused for the purpose of engraftment (ie, bone marrow transplant recipients) may have their blood or bone marrow monitored for an estimate of the percentage of donor and recipient cells present. This can be done by identifying unique features of the donor's and the recipient's DNA prior to transplantation and then examining the recipient's blood or bone marrow after the transplantation procedure has occurred. The presence of both donor and recipient cells (chimerism) and the percentage of donor cells are indicators of transplant success.

 

Short tandem repeat (STR) sequences are used as identity markers. STR are di-, tri-, or tetra-nucleotide repeat sequences interspersed throughout the genome at specific sites. There is variability in STR length among people, and the STR lengths remain stable throughout life, making them useful as identity markers. Polymerase chain reaction is used to amplify selected STR regions from germline DNA of both donor and recipient. The lengths of the amplified fragment are evaluated for differences (informative markers). Following allogeneic hematopoietic cell infusion, the recipient blood or bone marrow can be evaluated again for the informative STR regions to identify chimerism and estimate the proportions of donor and recipient cells in the specimen.

Reference Values

An interpretive report will be provided.

Interpretation

An interpretive report will be provided, which defines unique features of the donor's cells.

 

It is most useful to observe a trend in chimerism levels. Clinically critical results should be confirmed with 1 or more subsequent specimens.

Clinical Reference

1. Antin JH, Childs R, Filipovich AH, et al: Establishment of complete and mixed donor chimerism after allogenic lymphohematopoietic transplantation: recommendations from a workshop at the 2001 Tandem Meetings of the International Bone Marrow Transplant Registry and the American Society of Blood and Bone Marrow Transplantation. Biol Blood Marrow Transplant. 2001;7(9):473-485

2. Tang X, Alatrash G, Ning J, et al: Increasing chimerism following allogeneic stem cell transplantation is associated with longer survival time. Biol Blood Marrow Transplant. 2014 Aug;20(8):1139-1144. doi: 10.1016/j.bbmt.2014.04.003

3. Ludeman MJ, Zhong C, Mulero JJ, et al: Developmental validation of GlobalFiler PCR amplification kit: a 6-dye multiplex assay designed for amplification of casework samples. Int J Legal Med. 2018 Nov;132(6):1555-1573. doi: 10.1007/s00414-018-1817-5

4. Tyler J, Kumer L, Fisher C, Casey H, Shike H: Personalized chimerism test that uses selection of short tandem repeat or quantitative PCR depending on patient's chimerism status. J Mol Diagn. 2019 May;21(3):483-490. doi: 10.1016/j.jmoldx.2019.01.007

5. Lion T, Watzinger F, Preuner S, et al: The EuroChimerism concept for a standardized approach to chimerism analysis after allogeneic stem cell transplantation. Leukemia. 2012 Aug;26(8):1821-1828. doi: 10.1038/leu.2012.66

Report Available

4 to 8 days following receipt of Pre and Donor Specimens

Test Classification

This test was developed and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. It has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81267-Chimerism (engraftment) analysis, post hematopoietic stem cell transplantation specimen, includes comparison to previously performed baseline analyses, without cell selection

LOINC Code Information

Test ID Test Order Name Order LOINC Value
CHIMU Chimerism Transplant No Cell Sort 34574-4

 

Result ID Test Result Name Result LOINC Value
MP026 Specimen Type 31208-2
37313 Final Diagnosis 34574-4

Forms

1. Chimerism Analysis Information Sheet (T594)

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Day(s) Performed

Monday through Friday

Mayo Clinic Laboratories | Hematology Catalog Additional Information:

mml-bone-marrow-transplant