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Test ID: NGSMM Multiple Myeloma Gene Panel, Next-Generation Sequencing, Bone Marrow

Useful For

Evaluation of multiple myeloma at the time of diagnosis, for prognostic and potential therapeutic indications


Identification of the presence of new, clinically important, gene alteration changes at relapse

Testing Algorithm

See Targeted Genes Interrogated by Multiple Myeloma Next-Generation Sequencing in Special Instructions for a list of the genes and exons targeted by this assay.

Method Name

Next-Generation Sequencing (NGS)

Reporting Name

NGS Multiple Myeloma

Specimen Type

Bone Marrow

Shipping Instructions

Ship samples Monday through Friday

Necessary Information

The following information is required:

1. Clinical diagnosis

2. Pertinent clinical history

3. Clinical or morphologic suspicion

4. Date of collection

5. Specimen source

Specimen Required

Specimen Type: Bone marrow aspirate

Container/Tube: Lavender top (EDTA) or yellow top (ACD)

Specimen Volume: 2 mL

Collection Instructions:

1. Invert several times to mix bone marrow.

2. Send specimen in original tube.

3. Label specimen as bone marrow.

4. Fresh specimen is required for this test, as testing is performed on sorted cells.

Specimen Minimum Volume

1 mL

Specimen Stability Information

Specimen Type Temperature Time Special Container
Bone Marrow Ambient 4 days PURPLE OR PINK TOP/EDTA

Clinical Information

Multiple myeloma (MM) is a malignancy of bone marrow plasma cells with an annual incidence of 200,000 per annum. Comprehensive clinical, radiologic, and laboratory evaluation can initially stratify patients by disease phase and burden. Cytogenetic and FISH studies are important to help classify MM into standard, intermediate, and high risk groups. Advances in nontargeted therapies, including autologous bone marrow transplantation, have significantly improved the outcome of many patients; however, most patients with myeloma suffer relapse after initial treatment. Clinical next-generation sequencing (NGS) technology has enabled a deeper and more detailed evaluation of MM genetics. Testing allows for further risk categorization of the disease through the identification of additional abnormalities of prognostic and potentially therapeutic value. Application of targeted NGS-based analysis is a useful adjunct to the standard evaluation of MM patients at diagnosis and relapse. This test comprises a DNA-based multigene panel that includes preanalytic plasma cell enrichment, NGS, and detailed analysis resulted in a clinical report.

Reference Values

An interpretive report will be provided.


An interpretive report will be provided that includes the gene alterations identified, if present.

Clinical Reference

1. Walker BA, Boyle, EM, Wardell CP, et al: Mutational spectrum, copy number changes, and outcome: results of a sequencing study of patients with newly diagnosed myeloma. J Clin Oncol 2015;33:3911-3920

2. Morgan GJ, Walker BA, Davies FE: The genetic architecture of multiple myeloma. Nat RevCancer. 2012;12(5):335-348

3. Kortuem KM, Braggio E, Bruins L, et al: Panel sequencing for clinically oriented variant screening and copy number detection in 142 untreated multiple myeloma patients. Blood Cancer J. 2016;6:e397

4. Kortuem KM, Mai EK, Hanafiah NH, et al: Targeted sequencing of refractory myeloma reveals a high incidence of mutations in CRBN and Ras pathway genes. Blood 2016;128:1226-1233

Day(s) Performed


Report Available

14 to 21 days

Test Classification

This test was developed, and its performance characteristics determined by Mayo Clinic in a manner consistent with CLIA requirements. This test has not been cleared or approved by the US Food and Drug Administration.

CPT Code Information

81455-Targeted genomic sequence analysis panel, solid organ or hematolymphoid neoplasm, DNA and RNA analysis when performed, 51 or greater genes (eg, ALK, BRAF, CDKN2A, CEBPA, DNMT3A, EGFR, ERBB2, EZH2, RLT3, IDH1, IDH2, JAK2, KIT, KRAS, MLL, NPM1, NRAS, MET, NOTCH1, PDGRA, PDGFRB, PGR, PIK3CA, PTEN, RET), interrogation for sequence variants and copy number variants or rearrangements, if performed.

LOINC Code Information

Test ID Test Order Name Order LOINC Value
NGSMM NGS Multiple Myeloma In Process


Result ID Test Result Name Result LOINC Value
43553 NGSMM Result No LOINC Needed
43478 Pathogenic Mutations Detected 41103-3
43477 Interpretation 69047-9
43479 Clinical Trials 82786-5
43480 Variants of Unknown Significance 93367-1
43481 Additional Notes 48767-8
43482 Method Summary 85069-3
43483 Disclaimer 62364-5
43484 NGSMM Panel Gene list 36908-2
43485 Reviewed By 19139-5


1. Hematopathology Patient Information (T676) in Special Instructions

2. If not ordering electronically, complete, print, and send a Hematopathology/Cytogenetics Test Request (T726) with the specimen.

Additional Tests

Test ID Reporting Name Available Separately Always Performed
CSNMM NGSMM Pre-Analysis Cell Sorting, BM No Yes
Mayo Clinic Laboratories | Hematology Catalog Additional Information: